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|118||Muscle Specific MicroRNAs are regulated by Endurance Exercise in Human Skeletal Muscle.|
Nielsen S; Scheele C; Yfanti C; Akerstrom TC; Nielsen AR; Pedersen BK; Laye MJ
J Physiol 2010; 588(20): 4029-37
PubMed ID: 20724368
Muscle specific miRNAs, myomiRs, have been shown to control muscle development in vitro and are differentially expressed at rest in diabetic skeletal muscle. Therefore, we investigated the expression of these myomiRs, including miR-1, miR-133a, miR-133b and miR-206 in muscle biopsies from vastus lateralis of healthy young males (n=10) in relation to a hyperinsulinemic-euglycemic clamp as well as acute endurance exercise before and after 12 weeks of endurance training. The subjects increased their endurance capacity, VO2max (l/min) with 17.4% (P<0.001), and improved insulin sensitivity by 19% (P<0.01). While myomiR expression remained stable during a hyperinsulinemic-euglycemic clamp, an acute bout of exercise increased mir-1 (P<0.05) and mir-133a (P<0.05) expression before, but not after, training. In resting biopsies, endurance training for 12 weeks decreased basal expression of all four myomiRs (P<0.05). Interestingly, all myomiRs were reverted to their pre-training expression levels 14 days after ceasing the training program. Major pathways involved in endurance adaptation such as MAPK and TGF-beta were predicted to be targeted by the examined myomiRs.Tested predicted target proteins included Cdc42 and ERK 1/2. Although these proteins were downregulated between post training period and 2 weeks of cessation, an inverse correlation between myomiR and target proteins did not exist. In conclusion, our data suggest myomiRs respond to physiological stimuli, but their role in regulating human skeletal muscle adaptation remains unknown.