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|175||In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue.|
Hojman P; Eriksen J; Gehl J
Biol Proced Online 2009; 11: 253-62
PubMed ID: 19495913
DNA electrotransfer to muscle tissue yields long-term, high levels of gene expression; showing great promise for future gene therapy. We want to characterize the novel far-red fluorescent protein Katushka as a marker for gene expression using time domain fluorescence in vivo imaging. Highly efficient transgenic expression was observed after DNA electrotransfer with 100-fold increase in fluorescent intensity. The fluorescent signal peaked 1 week after transfection and returned to background level within 4 weeks. Katushka expression was not as stable as GFP expression, which was detectable for 8 weeks. Depth and 3D analysis proved that the expression was located in the target muscle. In vivo bio-imaging using the novel Katushka fluorescent protein enables excellent evaluation of the transfection efficacy, and spatial distribution, but lacks long-term stability. Hojman, Pernille Eriksen, Jens Gehl, Julie eng Journal Article 20090410 England